Heavner, Benjamin D and Smallbone, Kieran and Barker, Brandon and Mendes, Pedro and Walker, Larry P (2012) Yeast 5 � an Expanded Reconstruction of the Saccharomyces Cerevisiae Metabolic Network. BMC Systems Biology. (In Press)
![[thumbnail of heavner12.pdf]](https://eprints.maths.manchester.ac.uk/style/images/fileicons/application_pdf.png) |
PDF
heavner12.pdf Download (415kB) |
Abstract
Background: Efforts to improve the computational reconstruction of the Saccharomyces cerevisiae biochemical reaction network and to refine the stoichiometrically constrained metabolic models that can be derived from such a reconstruction have continued since the first stoichiometrically constrained yeast genome scale metabolic model was published in 2003. Continuing this ongoing process, we have constructed an update to the Yeast Consensus Reconstruction, Yeast 5. The Yeast Consensus Reconstruction is a product of efforts to forge a community-based reconstruction emphasizing standards compliance and biochemical accuracy via evidence-based selection of reactions. It draws upon models published by a variety of independent research groups as well as information obtained from biochemical databases and primary literature. Results: Yeast 5 refines the biochemical reactions included in the reconstruction, particularly reactions involved in sphingolipid metabolism; updates gene-reaction annotations; and emphasizes the distinction between reconstruction and stoichiometrically constrained model. Although it was not a primary goal, this update also improves the accuracy of model prediction of viability and auxotrophy phenotypes and increases the number of epistatic interactions. This update maintains an emphasis on standards compliance, unambiguous metabolite naming, and computer-readable annotations available through a structured document format. Additionally, we have developed MATLAB scripts to evaluate the model�s predictive accuracy and to demonstrate basic model applications, such as simulating aerobic and anaerobic growth. These scripts, which provide an independent tool for evaluating the performance of various stoichiometrically constrained yeast metabolic models using flux balance analysis, are included as additional files. Conclusions: Yeast 5 expands and refines the computational reconstruction of yeast metabolism and improves the predictive accuracy of a stoichiometrically constrained yeast metabolic model. It differs from previous reconstructions and models by emphasizing the distinction between the yeast metabolic reconstruction and the stoichiometrically constrained model, and makes both available as additional files and at http://yeast.sf.net/ as separate systems biology markup language (SBML) files. Through this separation, we intend to make the modeling process more accessible, explicit, transparent, and reproducible.
| Item Type: | Article |
|---|---|
| Subjects: | MSC 2010, the AMS's Mathematics Subject Classification > 92 Biology and other natural sciences |
| Depositing User: | Dr Kieran Smallbone |
| Date Deposited: | 06 May 2012 |
| Last Modified: | 20 Oct 2017 14:13 |
| URI: | https://eprints.maths.manchester.ac.uk/id/eprint/1822 |
Available Versions of this Item
- Yeast 5 � an Expanded Reconstruction of the Saccharomyces Cerevisiae Metabolic Network. (deposited 06 May 2012) [Currently Displayed]
Actions (login required)
 |
View Item |